As they have a phagocytic function, removing pathogens and cell debris, macrophages usually contain abundant primary and secondary lysosomes. It is made up of type II collagen and is located in the auricle of the ear and the epiglottis. Large amounts of rough ER indicate that the cell is active and is producing large amounts of proteins. Macrophages can be seen also in the subcapsular sinus (the lighter staining area just under the capsule at the periphery of the lymph node). Correct answer 4. It is comprised primarily of type II collagen. Loose Connective Tissue. Look for a region characterized by interlacing cords of cells. Neutrophils and macrophages are also present and both are discussed below. They are especially common along smaller blood vessels. Study Microscopic Images of Tissues Flashcards Flashcards at ProProfs - This set of image-based flashcards gives medical students the Microscopic Images of Tissues. The collagen of fibrous supporting tissues, the dermis of the skin, tendon, ligaments and bone is type I collagen, which provides tensile strength. It is significantly weaker in individuals with Marfan syndrome. What are the secretory products of the mast cell? The antibody binds mast cells and waits for a second exposure to whatever it happens to be responsive to (an allergen). In contrast to epithelia, connective tissue is sparsely populated by cells and contains an extensive extracellular matrix consisting of protein fibers, glycoproteins, and proteoglycans. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. What functions does this type of connective tissue play to support the epithelium? What is their function. Aug 30, 2014 - This Pin was discovered by Haley Boyer. Muscle tissue is specialized for contraction. As fibroblasts are the most common cells in areolar tissue, the majority of the nuclei seen here are probably fibroblast nuclei. (CT7), 18 Loose Connective Tissue - Lamina propria of tracheal mucosa View Virtual EM Slide In addition to some fibroblasts and a few delicate collagen fibers, you should see quite a few plasma cells View Image amongst the epithelial tubes. Slide 36 is stained with aldehyde fuchsin and Masson trichrome (Aldehyde Fuchsin, Fe. Allow wall of artery to expand and contract. This is loose connective tissue. Loose (areolar connective tissue) is the most abundant form of collagenous connective tissue. Note the coarse chromatin clumps organzed as radial spokes in the round nuclei which is therefore often described as having a "clockface" or "wagon-wheel" appearance. Note at high power that fibrils or fibers of any type cannot be readily observed. Connective tissue is divided into four main categories: Connective proper; Cartilage; Bone; Blood; Connective tissue proper has two subclasses: loose and dense. Connective Tissue: Connective tissue is found in adipose, bone, ligaments, tendons, nerves, cartilage and muscles. Lacunae are separated from one another as a result of the secretory activity of the chondrocytes. The collagen fibers (cf) are parallel to the arrow bar. Adipose or fat tissue has no visible fibers. See more ideas about tissue, things under a microscope, fun facts. Slide 106 and 112 have bits of well preserved flexor tendon at the top of the section (the tissue at the very top of slide 112 is actually skeletal muscle -which you'll study in the next lab; the tendon is just below it). Be able to use knowledge about the physical characteristics of collagen and elastin in explaining the functions of tissue where these molecules occur in large quantities (, Be able to recognize different types of connective tissue (. Identify the tissue depicted in the below micrograph. slide 36 (Aorta, aldehyde fuchsin) View Virtual Slide. Fibroblasts are by far the most common native cell type of connective tissue. These cells make a large amount of protein that they secrete to build the connective tissue layer. Most frequently, the different types of connective tissues are specified by their content of three distinguishing types of extracellular fibers: collagenous fibers, elastic fibers, and reticular fibers. Stratified squamous non-keratinizing epithelium lines the pharynx. You should be able to recognize a range of nuclear morphologies and be able to identify the cells as fibroblasts. Attempt and answer these flashcards quizzes easily and have a smooth … Discover (and save!) Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. Make sure you can see the difference between cross sectioned and longitudinally sectioned collagenous fibrils. White fat cells or adipocytes are specialized for the storage of triglyceride, and occur singly or in small groups scattered throughout the loose connective tissue. The fibroblast synthesizes the collagen and ground substance of the extracellular matrix. Two other locations where elastic fibers can be readily seen in H&E sections are in the lamina propria of the pharynx (slide 152 View Image ) and the trachea (slide 40 View Image ). It is significantly weaker in individuals with Marfan syndrome. Please read the descriptions of the connective tissue stains BEFORE you do your lab work. You can see one large lipid droplet in the cytoplasm of each cell. The English word "tissue" derives from the French word "tissue", meaning that … A collagen fibril -the brackets enclose a single collagen FIBRIL, which is made of 1000s of tropocollagen units attached in a head-to-tail arrangement. Individual lacunae may contain multiple cells deriving from a common progenitor. Note that the nucleoplasm of a fibroblast has a generally fine stippled (dot-like) chromatin pattern with occasional coarse chromatin clumps (heterochomatin) and one or two nucleoli. Both are fluid, … "loose" versus "dense" connective tissue), sometimes it is not always possible to classify connective tissue in a given section; it may be a little loose, a little dense, a little fatty, etc. Slide 29 (small intestine, H&E) View Virtual Slide. None of our slides of adult tissue shows any brown fat, however this rather unique tissue can be seen in slide H2 View Image, which is from a developing fetus. Mast cells can only be definitively recognized with special stains such as Azure II and toluoidine blue that identify the heparin storage granules (Azure metachromatically stains the heparin purple). As you look at this section, you will see a very obvious layer of mucous epithelial cells (PAS also reacts with the carbohydrate-rich mucin). Brown fat cells are highly specialized for temperature regulation. These are termed reticular (or rarely, argyrophilic= "silver loving") fibers. Many of the free cells in these medullary sinuses cannot be identified; however, the large rounded cells, with eccentrically placed, vesicular nuclei are the ones you should try to find. This cartilage is found in the nose, tracheal rings, and where the ribs join the sternum. Important cells found in the loose connective tissue include the following: Fibroblasts, which synthesize collagenous connective tissue fibers that are flexible but of great tensile strength; macrophages (or histiocytes) and monocytes, which ingest, digest, or "store" microscopic particles such as debris of dead cells; certain microorganisms; and other non-biodegradable … Unlike the tissue of the aorta which would be classified as "regular," these tissues are obviously irregular but they're mentioned here for the purpose of illustrating how aggregates of elastic fibers appear in H&E-stained sections. Find the Golgi complex, a pale or slightly eosinophilic (=eosin "loving", an area rich in membranes containing basic amino acids, syn. 65-81, Supporting/Connective tissues, Ross and Pawlina (6th ed), Chapter 6 Connective Tissue, pgs. Connective tissue is made up of cells and extracellular matrix. Chapter 3 - Connective Tissue. Describe the structural organization of the fibers in the extracellular matrix and the cells residing within connective tissue, Distinguish loose and dense connective tissue using the light microscope, Describe the structure and function of cartilage. The degranulation of these cells is responsible for triggering type I, immediate hypersensitivity reactions. Finally, recall that PROcollagen is INTRAcellular and doesn't aggregate into fibrils as shown here until secreted. Connective tissue is a type of animal tissue made up of cells, fibers, and gel-like substances that supports and gives structure to the body. NOTE:  Slide 250 illustrates a point about the limits of classification schemes. In dense connective tissue, which type of cell is most common? Note the relative size of the different cell types, their shapes, amount of rough ER and variously sized granules and inclusions. Mast cells are actively involved in a host's immune response and produce many substances, some of which are heparin and histamine. 2. Multiple fibrils are bundled together to form a FIBER (which is what you can see in the light microscope). Look for adipose tissue in Slide 30 which is taken from abdominal mesentery (the connective tissue that suspends the viscera within the abdominal cavity). Look for elongated nuclei, usually solitary, from which a modest amount of tapered cytoplasm extends from either one or both poles of the nucleus. Dense regular connective tissue 400X Tendon In this image a fibroblast nucleus (fb nuc) is labeled, but you can see other nuclei once you know what to look for. Many of the major types of cellular organelles are visible in this image. The Golgi complex in these particular plasma cells is usually in the form of a fine crescent adjacent to the nucleus and it takes some practice to recognize. 28 Collagen and Elastin - Cross section of Chorda Tendinea View Virtual EM Slide 1. In biology, tissue is a cellular organizational level between cells and a complete organ.A tissue is an ensemble of similar cells and their extracellular matrix from the same origin that together carry out a specific function. Macrophages phagocytose foreign material in the connective tissue layer and also play an important role as antigen presenting cells, a function that you will learn more about in Immunobiology. Overview of Connective Tissue. In the region immediately beneath the epidermis you can see how the elastic fibers are interconnected forming an elaborate, delicate net of fibers View Image amongst thin strands of collagen. (CT11). For connective tissue stains – Van Gieson stains and variants. Then use your text and atlas to review the diagnostic features of each connective tissue cell present in the micrograph. The "mononuclear phagocyte system" (also called the "reticuloendothelial system" for historic reasons) consists of free and fixed macrophages throughout the body. The function of this type of tissue is to provide structural and mechanical support for other tissues, and to mediate the exchange of nutrients and waste between the circulation … Note the alternating layers of fibroblasts and collagenous fiber bundles. 26 Connective Tissue - Dense Irregular View Virtual EM Slide Tissue macrophages can be found in many different organs. A fatty tissue called brown or multilocular fat, produced during fetal development, has adipocytes that contain multiple fat droplets. Look for plasma cells within the lamina propria of slide 29 View Image. Be able to describe the functions of cells commonly found in connective tissue and identify them. You will study neutrophils in much greater detail in other sequences and in your histopathology course, but it is useful for now to at least be able to recognize them in various tissues and organs. Although collagen fibers mostly fill the view, there are numerous elastic fibers, which provide the elasticity essential for the function of the tissue. The cell nucleus is flat and oval. Usually there is more extracellular matrix than cellular material. Dense regular connective tissue - the tissue shown has a dense, regular arrangement of collagen fibers; the cells present are fibroblasts NOT squamous epithelial cells. Resists tension in one direction. The area beneath the stratified squamous epithelium shown in slide 33 is the dermis, which is composed of dense irregular connective tissue. Today's Rank--0. They release histamine during allergic reactions. your own Pins on Pinterest Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. The granules are often so dark that they obscure the nucleus. Dense connective tissue is enriched in collagen fibers with little ground substance. Just as in the H&E-stained aorta, the elastic fibers in the pharynx and trachea are glassy and orange-red --they appear as stippled dots because they’ve been cut in cross section. This system consists of a number of tissue-specific, mobile, phagocytic cells that descend from monocytes - these include the Kupffer cells of the liver, the alveolar macrophages of the lung, the microglia of the central nervous system, and the reticular cells of the spleen. This generates heat. You should also observe that there aren't a lot of cells, a characteristic of "dense" connective tissue. Start studying Lab 4: Connective Tissue Microscope Slides. It is characterized by a prominent extracellular matrix consisting of various proportions of connective tissue fibers embedded in a gel-like matrix. Loose (areolar connective tissue) is the most abundant form of collagenous connective tissue. Wheater's, pgs. Observe the mixture of collagen and elastic fibers in this cross section of chorda tendinea. Because of their nuclear morphology, they are frequently also called “polymorphonuclear leukocytes” (aka "PMNs" or “polys”). When we study smooth muscle and peripheral nerve tissue we will come back to this slide to try and distinguish between collagen fibers and fascicles of smooth muscle and/or nerve fibers and ganglia. They phagocytose cell debris and/or foreign material. While epithelia cover and protect, connective tissues “connect” and store. Connective tissue cells are usually divided into two types: Fixed cells (or resident cells) - resident population of cells that develop and remain within connective tissue. Many of these macrophages contain phagocytosed red blood cells or the brownish breakdown pigment, hemosiderin (which is the result of lysosomal action on the ingested red blood cells.). Non-striated (involuntary) muscle E 5 Striated muscle, showing striations and nuclei LS 5 Adipose tissue, section 5 Pseudo-stratified ciliated: epithelium TS 5 Areolar tissue, stained for fibres and cells E 5 Spinal cord TS 5 Compact bone, for Haversian Canals and lamellae 5 Cardiac muscle showing the nuclei, striations and intercalated discs LS 5 Dense Connective Tissue. This slide has been stained with iron hematoxylin and eosin so you can see collagen fibers (orange) as well as elastic fibers (purple/black) in the dermis View Image. Note also that thebasement membrane underlying this particular epithelium is especially prominent. You should note that the cytoplasm is quite basophilic (i.e. However, deeper in the dermis, the collagen and elastic fibers are much thicker. 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